Melanie Kimm, PhD
Institut für diagnostische und interventionelle Radiologie
Klinikum rechts der Isar
Technische Universität München
Ismaningerstr. 22, 81675 München
Tel.: (089) 4140-6351
E-Mail: melanie.kimm@tum.de
Moritz Wildgruber MD, PhD
email: moritz.wildgruber@tum.de
My current research interest is focused on multimodality imaging of the infarcted myocardium and subsequent healing. In-vivo assessment of specific aspects of myocardial remodeling after ischemic injury can help to provide a more dynamic understanding of the healing myocardium and improve the monitoring and evaluation of novel therapy approach such as stem-cells. The major techniques used are Magnetic Resonance Imaging and optical imaging techniques as well as nuclear approaches. MRI yields excellent anatomic resolution and provides detailed information about cardiac function. Molecular MRI contrast agents, based on paramagnetic Gadolinium or superparamagnetic nanoparticles, can address specific targets in the injured myocardium such as extracellular matrix proteins and elastin fibers. Optical reporters can target proteolytic enzymes, immune cells such as monocytes and macrophages and surface proteins such as integrins or apoptosis markers.
While further development of in-vivo imaging approaches remains the central goal, the ex-vivo validation of the imaging signal is a crucial task to verify the cellular source of the in-vivo imaging signature. All projects carry a translational potential for clinical medicine.
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| MRI heart function | Molecular MRI (infarct healing) | FMT-CT (Apoptosis) |
Irina Eskina, Technician
Isabella Bielicki, MD student
Almut Glinzer, PhD student
Claudia Groß, Ph.D.
Laura Hoffmann, MD student
Dr. Katja Kosanke, Veterinarian
Dr. Fabian Lohoefer, Postdoc
Valerie Van Phi, MD student
René Botnar PhD, King's College London
Vasilis Ntziachristos PhD, Institute for biological and medical Imaging, Helmholtz Zentrum München and Technische Universität München
Axel Walch, MD, Research Unit Analytical Pathology, Helmholtz Zentrum München
Frank Siebenhaar MD, Allergie Centrum Charité, Humboldt Universität Berlin
Ralph Weissleder MD, PhD, Massachusetts General Hospital and Harvard Medical School
European Society of Cardiology: ESC TV 2014 - BASIC SCIENCE, 2014 Aug 30
Aichler M, Huber K, Schilling F, Lohöfer F, Kosanke K, Meier R, Rummeny EJ, Walch A, Wildgruber M. Spatially Resolved Quantification of Gadolinium (III)-Based Magnetic Resonance Agents in Tissue by MALDI Imaging Mass Spectrometry after In Vivo MRI. Angew Chem Int Ed Engl. 2015 Mar 27;54(14):4279-83
Wildgruber M, Bielicki I, Aichler M, Kosanke K, Feuchtinger A, Settles M, Onthank DC, Cesati RR, Robinson SP, Huber AM, Rummeny EJ, Walch AK, Botnar RM. Assessment of myocardial infarction and post infarction myocardial remodeling with an elastin-specific magnetic resonance agent. Circ Cardiovasc Imaging. 2014 Mar 1; 7(2):321-9
Ale A*, Siebenhaar F*, Kosanke K, Aichler M, Radrich K, Heydrich S, Schiemann M, Bielicki I, Noel PB, Braren R, Maurer M, Walch AK, Rummeny EJ, Ntziachristos V, Wildgruber M (2013). Cardioprotective c-kit+ bone marrow cells attenuate apoptosis after acute myocardial infarction in mice – in-vivo assessment with Fluorescence Molecular Imaging. Theranostics; 3(11):903-13
Wildgruber M, Swirski FK, Zernecke A (2013). Molecular Imaging of Inflammation in Atherosclerosis. Theranostics; 3(11):865-84
Wildgruber M, Settles M, Kosanke K, Bielicki I, Ntziachristos V, Rummeny EJ, Botnar RM, Huber AM (2012). Assessment of myocardial infarction in mice with a clinical Magnetic Resonance scanner – Comparison of phase-sensitive versus magnitude reconstructed inversion-recovery imaging. J Magn Reson Imaging; 36(6):1372-82
Settles M, Etzrodt M, Kosanke K, Schiemann M, Zimmermann A, Meier R, Braren R, Huber A, Rummeny EJ, Weissleder R, Swirski FK, Wildgruber M (2011). Different capacity of Monocyte Subsets to Phagocytose Iron-Oxide Nanoparticles. PLoS One; 6(10): e25197
Swirski FK, Wildgruber M, Ueno T, Figueiredo JL, Panizzi P, Iwamoto Y, Zhang E, Stone JR, Rodriguez E, Chen J, Pittet MJ, Weissleder R, Nahrendorf M (2010). Myeloperoxidase-rich Ly-6C+ myeloid cells infiltrate allografts and contribute to an imaging signature of organ rejection in mice. J Clin Invest; 120: 2627-34
Panizzi P, Nahrendorf M, Wildgruber M, Waterman P, Figueiredo JL, Aikawa E, McCarthy J, Weissleder R, Hilderbrand S (2009). Oxazine conjugated nanoparticle detects in vivo hypochlorous acid and peroxynitrite generation. J Am Chem Soc; 131(43): 15739-44
Swirski FK*, Nahrendorf M*, Etzrodt M, Wildgruber M, Cortez-Retamozo V, Panizzi P, Figueiredo JL, Kohler R, Chudnovskiy A, Waterman P, Aikawa E, Mempel TR, Libby P, Weissleder R, Pittet MJ (2009). Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science; 325(5940): 612-6
Wildgruber M, Lee H, Chudnovskiy A, Yoon TJ, Etzrodt M, Pittet MJ, Nahrendorf M, Croce K, Libby P, Weissleder R, Swirski FK (2009). Monocyte subset dynamics in human atherosclerosis can be profiled with magnetic nano-sensors. PLoS ONE 4(5): e5663
Christen T*, Nahrendorf M*, Wildgruber M, Swirski FK, Aikawa E, Waterman P, Shimizu K, Weissleder R, Libby P (2009). Molecular imaging of innate immune cell function in transplant rejection. Circulation 119(14): 1925-32
